Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_21_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_21_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 50165026
Paired-end alignments with a unique best hit 18663451
Pairs without alignments under any condition 26951097
Pairs that did not map uniquely 4550478
Genomic sequence context not extractable (edges of chromosomes) 815

Cytosine Methylation

Total C's analysed 663658625
Methylated C's in CpG context 10380882
Methylated C's in CHG context 523955
Methylated C's in CHH context 2065001
Methylated C's in Unknown context 4757
Unmethylated C's in CpG context 75400080
Unmethylated C's in CHG context 117225316
Unmethylated C's in CHH context 458063391
Unmethylated C's in Unknown context 453397
Percentage methylation (CpG context) 12.1%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4685083 original top strand
CTOT 4644136 complementary to original top strand
CTOB 4643796 complementary to original bottom strand
OB 4689621 original bottom strand

Deduplication

Alignments analysed 18662636
Unique alignments 16594940
Duplicates removed 2067696
Duplicated alignments were found at 1843565 different positions

Cytosine Methylation after Extraction

Total C's analysed 399488670
Methylated C's in CpG context 6100968
Methylated C's in CHG context 311854
Methylated C's in CHH context 1238820
Unmethylated C's in CpG context 45081646
Unmethylated C's in CHG context 69907149
Unmethylated C's in CHH context 276848233
Percentage methylation (CpG context) 11.9%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%

M-Bias Plot