Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_20_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_20_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 53149153
Paired-end alignments with a unique best hit 18173439
Pairs without alignments under any condition 30546046
Pairs that did not map uniquely 4429668
Genomic sequence context not extractable (edges of chromosomes) 687

Cytosine Methylation

Total C's analysed 646496659
Methylated C's in CpG context 10069414
Methylated C's in CHG context 516273
Methylated C's in CHH context 2038431
Methylated C's in Unknown context 4362
Unmethylated C's in CpG context 73466096
Unmethylated C's in CHG context 114151652
Unmethylated C's in CHH context 446254793
Unmethylated C's in Unknown context 410566
Percentage methylation (CpG context) 12.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4335552 original top strand
CTOT 4741093 complementary to original top strand
CTOB 4750989 complementary to original bottom strand
OB 4345118 original bottom strand

Deduplication

Alignments analysed 18172752
Unique alignments 16178353
Duplicates removed 1994399
Duplicated alignments were found at 1781652 different positions

Cytosine Methylation after Extraction

Total C's analysed 392050055
Methylated C's in CpG context 5957717
Methylated C's in CHG context 310331
Methylated C's in CHH context 1231939
Unmethylated C's in CpG context 44300730
Unmethylated C's in CHG context 68625359
Unmethylated C's in CHH context 271623979
Percentage methylation (CpG context) 11.9%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot