Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_19_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_19_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 48336315
Paired-end alignments with a unique best hit 18400790
Pairs without alignments under any condition 25394391
Pairs that did not map uniquely 4541134
Genomic sequence context not extractable (edges of chromosomes) 762

Cytosine Methylation

Total C's analysed 651759091
Methylated C's in CpG context 9988466
Methylated C's in CHG context 513314
Methylated C's in CHH context 2038370
Methylated C's in Unknown context 4305
Unmethylated C's in CpG context 74050719
Unmethylated C's in CHG context 115263696
Unmethylated C's in CHH context 449904526
Unmethylated C's in Unknown context 448902
Percentage methylation (CpG context) 11.9%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4616366 original top strand
CTOT 4578957 complementary to original top strand
CTOB 4584321 complementary to original bottom strand
OB 4620384 original bottom strand

Deduplication

Alignments analysed 18400028
Unique alignments 16515106
Duplicates removed 1884922
Duplicated alignments were found at 1685581 different positions

Cytosine Methylation after Extraction

Total C's analysed 393583006
Methylated C's in CpG context 5892422
Methylated C's in CHG context 306133
Methylated C's in CHH context 1222555
Unmethylated C's in CpG context 44447937
Unmethylated C's in CHG context 68992850
Unmethylated C's in CHH context 272721109
Percentage methylation (CpG context) 11.7%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%

M-Bias Plot