Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_18_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_18_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 50675924
Paired-end alignments with a unique best hit 19762420
Pairs without alignments under any condition 26011820
Pairs that did not map uniquely 4901684
Genomic sequence context not extractable (edges of chromosomes) 854

Cytosine Methylation

Total C's analysed 702187735
Methylated C's in CpG context 11122944
Methylated C's in CHG context 563351
Methylated C's in CHH context 2209287
Methylated C's in Unknown context 4640
Unmethylated C's in CpG context 79862407
Unmethylated C's in CHG context 125026145
Unmethylated C's in CHH context 483403601
Unmethylated C's in Unknown context 474810
Percentage methylation (CpG context) 12.2%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4968187 original top strand
CTOT 4901333 complementary to original top strand
CTOB 4914664 complementary to original bottom strand
OB 4977382 original bottom strand

Deduplication

Alignments analysed 19761566
Unique alignments 17635969
Duplicates removed 2125597
Duplicated alignments were found at 1897364 different positions

Cytosine Methylation after Extraction

Total C's analysed 418432386
Methylated C's in CpG context 6485534
Methylated C's in CHG context 331791
Methylated C's in CHH context 1308830
Unmethylated C's in CpG context 47232125
Unmethylated C's in CHG context 73829736
Unmethylated C's in CHH context 289244370
Percentage methylation (CpG context) 12.1%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.5%

M-Bias Plot