Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_18_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_18_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 50675924
Paired-end alignments with a unique best hit 19762420
Pairs without alignments under any condition 26011820
Pairs that did not map uniquely 4901684
Genomic sequence context not extractable (edges of chromosomes) 854
39%51.3%9.67%0.00169%
Unique AlignmentsNo AlignmentMultiple AlignmentsNo Genomic Sequence

Cytosine Methylation

Total C's analysed 702187735
Methylated C's in CpG context 11122944
Methylated C's in CHG context 563351
Methylated C's in CHH context 2209287
Methylated C's in Unknown context 4640
Unmethylated C's in CpG context 79862407
Unmethylated C's in CHG context 125026145
Unmethylated C's in CHH context 483403601
Unmethylated C's in Unknown context 474810
Percentage methylation (CpG context) 12.2%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%
CpG contextCHG contextCHH context020406080100
% Methylation

Alignment to Individual Bisulfite Strands

OT 4968187 original top strand
CTOT 4901333 complementary to original top strand
CTOB 4914664 complementary to original bottom strand
OB 4977382 original bottom strand
OTCTOTCTOBOB01M2M3M4M5M
Number of Alignments

Deduplication

Alignments analysed 19761566
Unique alignments 17635969
Duplicates removed 2125597
Duplicated alignments were found at 1897364 different positions
89.2%10.8%
Unique AlignmentsDuplicate Alignments

Cytosine Methylation after Extraction

Total C's analysed 418432386
Methylated C's in CpG context 6485534
Methylated C's in CHG context 331791
Methylated C's in CHH context 1308830
Unmethylated C's in CpG context 47232125
Unmethylated C's in CHG context 73829736
Unmethylated C's in CHH context 289244370
Percentage methylation (CpG context) 12.1%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.5%
CpG contextCHH contextCHG context020406080100
% Methylation

M-Bias Plot

204060801000204060801000.5M1M1.5M2M
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 1Position in Read [bp]% Methylation# Methylation Calls

2040608010002040608010000.2M0.4M0.6M0.8M1M1.2M
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 2Position in Read [bp]% Methylation# Methylation Calls