Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_17_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_17_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 57127374
Paired-end alignments with a unique best hit 21998260
Pairs without alignments under any condition 29217690
Pairs that did not map uniquely 5911424
Genomic sequence context not extractable (edges of chromosomes) 949

Cytosine Methylation

Total C's analysed 784014626
Methylated C's in CpG context 12262703
Methylated C's in CHG context 633324
Methylated C's in CHH context 2500503
Methylated C's in Unknown context 5428
Unmethylated C's in CpG context 90085935
Unmethylated C's in CHG context 139670160
Unmethylated C's in CHH context 538862001
Unmethylated C's in Unknown context 536395
Percentage methylation (CpG context) 12.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5508925 original top strand
CTOT 5478994 complementary to original top strand
CTOB 5489780 complementary to original bottom strand
OB 5519612 original bottom strand

Deduplication

Alignments analysed 21997311
Unique alignments 19345522
Duplicates removed 2651789
Duplicated alignments were found at 2313416 different positions

Cytosine Methylation after Extraction

Total C's analysed 460296504
Methylated C's in CpG context 7051331
Methylated C's in CHG context 367618
Methylated C's in CHH context 1456363
Unmethylated C's in CpG context 52422274
Unmethylated C's in CHG context 81263551
Unmethylated C's in CHH context 317735367
Percentage methylation (CpG context) 11.9%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot