Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_16_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_16_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 50134077
Paired-end alignments with a unique best hit 19218821
Pairs without alignments under any condition 25898695
Pairs that did not map uniquely 5016561
Genomic sequence context not extractable (edges of chromosomes) 779

Cytosine Methylation

Total C's analysed 672652797
Methylated C's in CpG context 10613837
Methylated C's in CHG context 526214
Methylated C's in CHH context 2064235
Methylated C's in Unknown context 4433
Unmethylated C's in CpG context 76566493
Unmethylated C's in CHG context 119341117
Unmethylated C's in CHH context 463540901
Unmethylated C's in Unknown context 451024
Percentage methylation (CpG context) 12.2%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4811418 original top strand
CTOT 4787632 complementary to original top strand
CTOB 4799974 complementary to original bottom strand
OB 4819018 original bottom strand

Deduplication

Alignments analysed 19218042
Unique alignments 16957597
Duplicates removed 2260445
Duplicated alignments were found at 2000998 different positions

Cytosine Methylation after Extraction

Total C's analysed 382781966
Methylated C's in CpG context 5910985
Methylated C's in CHG context 296157
Methylated C's in CHH context 1171795
Unmethylated C's in CpG context 43275050
Unmethylated C's in CHG context 67314545
Unmethylated C's in CHH context 264813434
Percentage methylation (CpG context) 12.0%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%

M-Bias Plot