Bismark report for: /gscratch/srlab/sr320/data/cg/zr3644_14_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_14_R2.fastp-trim.20201206.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/Crassostrea_gigas.oyster_v9.dna_sm.toplevel/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 36184563 Number of paired-end alignments with a unique best hit: 13543064 Mapping efficiency: 37.4% Sequence pairs with no alignments under any condition: 19378814 Sequence pairs did not map uniquely: 3262685 Sequence pairs which were discarded because genomic sequence could not be extracted: 540 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3381915 ((converted) top strand) GA/CT/CT: 3386033 (complementary to (converted) top strand) GA/CT/GA: 3387373 (complementary to (converted) bottom strand) CT/GA/GA: 3387203 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 480027296 Total methylated C's in CpG context: 8480484 Total methylated C's in CHG context: 392938 Total methylated C's in CHH context: 1533847 Total methylated C's in Unknown context: 3945 Total unmethylated C's in CpG context: 53333962 Total unmethylated C's in CHG context: 84692824 Total unmethylated C's in CHH context: 331593241 Total unmethylated C's in Unknown context: 320465 C methylated in CpG context: 13.7% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.2% Bismark completed in 0d 1h 45m 16s