Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_13_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_13_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 52950721
Paired-end alignments with a unique best hit 19985416
Pairs without alignments under any condition 28114199
Pairs that did not map uniquely 4851106
Genomic sequence context not extractable (edges of chromosomes) 903

Cytosine Methylation

Total C's analysed 714200604
Methylated C's in CpG context 11090841
Methylated C's in CHG context 566665
Methylated C's in CHH context 2241192
Methylated C's in Unknown context 4824
Unmethylated C's in CpG context 80665715
Unmethylated C's in CHG context 125693566
Unmethylated C's in CHH context 493942625
Unmethylated C's in Unknown context 499338
Percentage methylation (CpG context) 12.1%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5017886 original top strand
CTOT 4965872 complementary to original top strand
CTOB 4976476 complementary to original bottom strand
OB 5024279 original bottom strand

Deduplication

Alignments analysed 19984513
Unique alignments 17662731
Duplicates removed 2321782
Duplicated alignments were found at 2056618 different positions

Cytosine Methylation after Extraction

Total C's analysed 427676475
Methylated C's in CpG context 6502393
Methylated C's in CHG context 335205
Methylated C's in CHH context 1334605
Unmethylated C's in CpG context 47981015
Unmethylated C's in CHG context 74622637
Unmethylated C's in CHH context 296900620
Percentage methylation (CpG context) 11.9%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%

M-Bias Plot