Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_12_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_12_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 46634543
Paired-end alignments with a unique best hit 17658705
Pairs without alignments under any condition 24771302
Pairs that did not map uniquely 4204536
Genomic sequence context not extractable (edges of chromosomes) 742

Cytosine Methylation

Total C's analysed 621973155
Methylated C's in CpG context 8812032
Methylated C's in CHG context 461909
Methylated C's in CHH context 1882739
Methylated C's in Unknown context 4125
Unmethylated C's in CpG context 69583720
Unmethylated C's in CHG context 106686865
Unmethylated C's in CHH context 434545890
Unmethylated C's in Unknown context 446519
Percentage methylation (CpG context) 11.2%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4416453 original top strand
CTOT 4416920 complementary to original top strand
CTOB 4410252 complementary to original bottom strand
OB 4414338 original bottom strand

Deduplication

Alignments analysed 17657963
Unique alignments 15735538
Duplicates removed 1922425
Duplicated alignments were found at 1716901 different positions

Cytosine Methylation after Extraction

Total C's analysed 367870994
Methylated C's in CpG context 5088445
Methylated C's in CHG context 270615
Methylated C's in CHH context 1111798
Unmethylated C's in CpG context 40900863
Unmethylated C's in CHG context 62547662
Unmethylated C's in CHH context 257951611
Percentage methylation (CpG context) 11.1%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%

M-Bias Plot