Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_11_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_11_R2.fastp-trim.20201206.fq.gz

Data processed at 14:16 on 2021-02-17


Alignment Stats

Sequence pairs analysed in total 55732442
Paired-end alignments with a unique best hit 21036214
Pairs without alignments under any condition 29367085
Pairs that did not map uniquely 5329143
Genomic sequence context not extractable (edges of chromosomes) 975

Cytosine Methylation

Total C's analysed 745372684
Methylated C's in CpG context 11759767
Methylated C's in CHG context 583657
Methylated C's in CHH context 2301494
Methylated C's in Unknown context 5178
Unmethylated C's in CpG context 84865286
Unmethylated C's in CHG context 131980373
Unmethylated C's in CHH context 513882107
Unmethylated C's in Unknown context 515663
Percentage methylation (CpG context) 12.2%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5274769 original top strand
CTOT 5241321 complementary to original top strand
CTOB 5239540 complementary to original bottom strand
OB 5279609 original bottom strand

Deduplication

Alignments analysed 21035239
Unique alignments 18648722
Duplicates removed 2386517
Duplicated alignments were found at 2116154 different positions

Cytosine Methylation after Extraction

Total C's analysed 439858992
Methylated C's in CpG context 6792165
Methylated C's in CHG context 340767
Methylated C's in CHH context 1351971
Unmethylated C's in CpG context 49719278
Unmethylated C's in CHG context 77181294
Unmethylated C's in CHH context 304473517
Percentage methylation (CpG context) 12.0%
Percentage methylation (CHG context) 0.4%
Percentage methylation (CHH context) 0.4%

M-Bias Plot