---

Alignment Stats

Sequence pairs analysed in total 42084665 Paired-end alignments with a unique best hit 26054281 Pairs without alignments under any condition 10634728 Pairs that did not map uniquely 5395656 Genomic sequence context not extractable (edges of chromosomes) 3214

---

Cytosine Methylation

Total C's analysed 915596076 Methylated C's in CpG context 12606352 Methylated C's in CHG context 794454 Methylated C's in CHH context 3447794 Methylated C's in Unknown context 31626 Unmethylated C's in CpG context 103960711 Unmethylated C's in CHG context 159084684 Unmethylated C's in CHH context 635702081 Unmethylated C's in Unknown context 2260462 Percentage methylation (CpG context) 10.8% Percentage methylation (CHG context) 0.5% Percentage methylation (CHH context) 0.5% Methylated C's in Unknown context N/A%

---

Alignment to Individual Bisulfite Strands

OT 6507057 original top strand CTOT 6512024 complementary to original top strand CTOB 6514597 complementary to original bottom strand OB 6517389 original bottom strand

---

Deduplication

Alignments analysed 26051067 Unique alignments 23202668 Duplicates removed 2848399 Duplicated alignments were found at 2518293 different positions

---

Cytosine Methylation after Extraction

Total C's analysed 555970190 Methylated C's in CpG context 7498791 Methylated C's in CHG context 477736 Methylated C's in CHH context 2073193 Unmethylated C's in CpG context 62836784 Unmethylated C's in CHG context 95848491 Unmethylated C's in CHH context 387235195 Percentage methylation (CpG context) 10.7% Percentage methylation (CHG context) 0.5% Percentage methylation (CHH context) 0.5%

---

M-Bias Plot

---

---