Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_7_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_7_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 81002273
Paired-end alignments with a unique best hit 49862829
Pairs without alignments under any condition 21226484
Pairs that did not map uniquely 9912960
Genomic sequence context not extractable (edges of chromosomes) 6194

Cytosine Methylation

Total C's analysed 1758006995
Methylated C's in CpG context 23739641
Methylated C's in CHG context 1483719
Methylated C's in CHH context 6394648
Methylated C's in Unknown context 59090
Unmethylated C's in CpG context 197876058
Unmethylated C's in CHG context 302489755
Unmethylated C's in CHH context 1226023174
Unmethylated C's in Unknown context 4464600
Percentage methylation (CpG context) 10.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 12455478 original top strand
CTOT 12463867 complementary to original top strand
CTOB 12472451 complementary to original bottom strand
OB 12464839 original bottom strand

Deduplication

Alignments analysed 49856635
Unique alignments 44115943
Duplicates removed 5740692
Duplicated alignments were found at 5010336 different positions

Cytosine Methylation after Extraction

Total C's analysed 1073400308
Methylated C's in CpG context 14192196
Methylated C's in CHG context 895497
Methylated C's in CHH context 3867789
Unmethylated C's in CpG context 120186442
Unmethylated C's in CHG context 183148940
Unmethylated C's in CHH context 751109444
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot