Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_6_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_6_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 52883485
Paired-end alignments with a unique best hit 32927367
Pairs without alignments under any condition 13405823
Pairs that did not map uniquely 6550295
Genomic sequence context not extractable (edges of chromosomes) 3803

Cytosine Methylation

Total C's analysed 1161276812
Methylated C's in CpG context 15428548
Methylated C's in CHG context 983123
Methylated C's in CHH context 4234417
Methylated C's in Unknown context 37887
Unmethylated C's in CpG context 131035907
Unmethylated C's in CHG context 200625235
Unmethylated C's in CHH context 808969582
Unmethylated C's in Unknown context 2915905
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8241768 original top strand
CTOT 8228543 complementary to original top strand
CTOB 8222799 complementary to original bottom strand
OB 8230454 original bottom strand

Deduplication

Alignments analysed 32923564
Unique alignments 28907419
Duplicates removed 4016145
Duplicated alignments were found at 3503295 different positions

Cytosine Methylation after Extraction

Total C's analysed 697964845
Methylated C's in CpG context 9092790
Methylated C's in CHG context 584509
Methylated C's in CHH context 2524196
Unmethylated C's in CpG context 78430613
Unmethylated C's in CHG context 119686169
Unmethylated C's in CHH context 487646568
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot