Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_5_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_5_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 43865548
Paired-end alignments with a unique best hit 27107123
Pairs without alignments under any condition 11405348
Pairs that did not map uniquely 5353077
Genomic sequence context not extractable (edges of chromosomes) 3174

Cytosine Methylation

Total C's analysed 958009481
Methylated C's in CpG context 12788107
Methylated C's in CHG context 806586
Methylated C's in CHH context 3444067
Methylated C's in Unknown context 31665
Unmethylated C's in CpG context 107783343
Unmethylated C's in CHG context 165366872
Unmethylated C's in CHH context 667820506
Unmethylated C's in Unknown context 2405194
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6724227 original top strand
CTOT 6820833 complementary to original top strand
CTOB 6825668 complementary to original bottom strand
OB 6733221 original bottom strand

Deduplication

Alignments analysed 27103949
Unique alignments 24155648
Duplicates removed 2948301
Duplicated alignments were found at 2604598 different positions

Cytosine Methylation after Extraction

Total C's analysed 587256545
Methylated C's in CpG context 7677529
Methylated C's in CHG context 490345
Methylated C's in CHH context 2093840
Unmethylated C's in CpG context 65780729
Unmethylated C's in CHG context 100557046
Unmethylated C's in CHH context 410657056
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot