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Alignment Stats

Sequence pairs analysed in total 43865548 Paired-end alignments with a unique best hit 27107123 Pairs without alignments under any condition 11405348 Pairs that did not map uniquely 5353077 Genomic sequence context not extractable (edges of chromosomes) 3174

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Cytosine Methylation

Total C's analysed 958009481 Methylated C's in CpG context 12788107 Methylated C's in CHG context 806586 Methylated C's in CHH context 3444067 Methylated C's in Unknown context 31665 Unmethylated C's in CpG context 107783343 Unmethylated C's in CHG context 165366872 Unmethylated C's in CHH context 667820506 Unmethylated C's in Unknown context 2405194 Percentage methylation (CpG context) 10.6% Percentage methylation (CHG context) 0.5% Percentage methylation (CHH context) 0.5% Methylated C's in Unknown context N/A%

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Alignment to Individual Bisulfite Strands

OT 6724227 original top strand CTOT 6820833 complementary to original top strand CTOB 6825668 complementary to original bottom strand OB 6733221 original bottom strand

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Deduplication

Alignments analysed 27103949 Unique alignments 24155648 Duplicates removed 2948301 Duplicated alignments were found at 2604598 different positions

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Cytosine Methylation after Extraction

Total C's analysed 587256545 Methylated C's in CpG context 7677529 Methylated C's in CHG context 490345 Methylated C's in CHH context 2093840 Unmethylated C's in CpG context 65780729 Unmethylated C's in CHG context 100557046 Unmethylated C's in CHH context 410657056 Percentage methylation (CpG context) 10.5% Percentage methylation (CHG context) 0.5% Percentage methylation (CHH context) 0.5%

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M-Bias Plot

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