Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_4_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_4_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 49072984
Paired-end alignments with a unique best hit 30338916
Pairs without alignments under any condition 12631541
Pairs that did not map uniquely 6102527
Genomic sequence context not extractable (edges of chromosomes) 3414

Cytosine Methylation

Total C's analysed 1070441903
Methylated C's in CpG context 13837410
Methylated C's in CHG context 858667
Methylated C's in CHH context 3628265
Methylated C's in Unknown context 34230
Unmethylated C's in CpG context 121042462
Unmethylated C's in CHG context 183557048
Unmethylated C's in CHH context 747518051
Unmethylated C's in Unknown context 2705275
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7593867 original top strand
CTOT 7572761 complementary to original top strand
CTOB 7575480 complementary to original bottom strand
OB 7593394 original bottom strand

Deduplication

Alignments analysed 30335502
Unique alignments 26771374
Duplicates removed 3564128
Duplicated alignments were found at 3139919 different positions

Cytosine Methylation after Extraction

Total C's analysed 644822263
Methylated C's in CpG context 8163029
Methylated C's in CHG context 512304
Methylated C's in CHH context 2175829
Unmethylated C's in CpG context 72617046
Unmethylated C's in CHG context 109726981
Unmethylated C's in CHH context 451627074
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot