Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_3_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_3_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 38671370
Paired-end alignments with a unique best hit 23834102
Pairs without alignments under any condition 9976314
Pairs that did not map uniquely 4860954
Genomic sequence context not extractable (edges of chromosomes) 3175

Cytosine Methylation

Total C's analysed 839797939
Methylated C's in CpG context 11184116
Methylated C's in CHG context 696921
Methylated C's in CHH context 2994671
Methylated C's in Unknown context 27975
Unmethylated C's in CpG context 95232592
Unmethylated C's in CHG context 145108747
Unmethylated C's in CHH context 584580892
Unmethylated C's in Unknown context 2112098
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5938529 original top strand
CTOT 5965504 complementary to original top strand
CTOB 5979092 complementary to original bottom strand
OB 5947802 original bottom strand

Deduplication

Alignments analysed 23830927
Unique alignments 21355848
Duplicates removed 2475079
Duplicated alignments were found at 2188864 different positions

Cytosine Methylation after Extraction

Total C's analysed 517555320
Methylated C's in CpG context 6757693
Methylated C's in CHG context 425267
Methylated C's in CHH context 1827542
Unmethylated C's in CpG context 58425404
Unmethylated C's in CHG context 88750219
Unmethylated C's in CHH context 361369195
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot