Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_2_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_2_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 54652876
Paired-end alignments with a unique best hit 33644497
Pairs without alignments under any condition 14291848
Pairs that did not map uniquely 6716531
Genomic sequence context not extractable (edges of chromosomes) 4579

Cytosine Methylation

Total C's analysed 1196487856
Methylated C's in CpG context 15705909
Methylated C's in CHG context 997996
Methylated C's in CHH context 4266399
Methylated C's in Unknown context 38110
Unmethylated C's in CpG context 135203659
Unmethylated C's in CHG context 205835746
Unmethylated C's in CHH context 834478147
Unmethylated C's in Unknown context 3029131
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8419537 original top strand
CTOT 8406880 complementary to original top strand
CTOB 8404902 complementary to original bottom strand
OB 8408599 original bottom strand

Deduplication

Alignments analysed 33639918
Unique alignments 29375152
Duplicates removed 4264766
Duplicated alignments were found at 3695983 different positions

Cytosine Methylation after Extraction

Total C's analysed 732190594
Methylated C's in CpG context 9423631
Methylated C's in CHG context 603203
Methylated C's in CHH context 2583393
Unmethylated C's in CpG context 82265495
Unmethylated C's in CHG context 124967372
Unmethylated C's in CHH context 512347500
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot