Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_24_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_24_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 51969546
Paired-end alignments with a unique best hit 32529762
Pairs without alignments under any condition 12847097
Pairs that did not map uniquely 6592687
Genomic sequence context not extractable (edges of chromosomes) 4090

Cytosine Methylation

Total C's analysed 1150684834
Methylated C's in CpG context 15437924
Methylated C's in CHG context 985478
Methylated C's in CHH context 4199617
Methylated C's in Unknown context 36832
Unmethylated C's in CpG context 130997565
Unmethylated C's in CHG context 200494614
Unmethylated C's in CHH context 798569636
Unmethylated C's in Unknown context 2846602
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8126667 original top strand
CTOT 8130907 complementary to original top strand
CTOB 8136174 complementary to original bottom strand
OB 8131924 original bottom strand

Deduplication

Alignments analysed 32525672
Unique alignments 28614568
Duplicates removed 3911104
Duplicated alignments were found at 3414322 different positions

Cytosine Methylation after Extraction

Total C's analysed 693808996
Methylated C's in CpG context 9122530
Methylated C's in CHG context 587921
Methylated C's in CHH context 2515070
Unmethylated C's in CpG context 78555091
Unmethylated C's in CHG context 119895031
Unmethylated C's in CHH context 483133353
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot