Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_23_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_23_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 39675229
Paired-end alignments with a unique best hit 24755468
Pairs without alignments under any condition 9789751
Pairs that did not map uniquely 5130010
Genomic sequence context not extractable (edges of chromosomes) 3045

Cytosine Methylation

Total C's analysed 868749682
Methylated C's in CpG context 11617935
Methylated C's in CHG context 745874
Methylated C's in CHH context 3185422
Methylated C's in Unknown context 28365
Unmethylated C's in CpG context 99227869
Unmethylated C's in CHG context 151272350
Unmethylated C's in CHH context 602700232
Unmethylated C's in Unknown context 2145535
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6215083 original top strand
CTOT 6160141 complementary to original top strand
CTOB 6165384 complementary to original bottom strand
OB 6211815 original bottom strand

Deduplication

Alignments analysed 24752423
Unique alignments 21887129
Duplicates removed 2865294
Duplicated alignments were found at 2529569 different positions

Cytosine Methylation after Extraction

Total C's analysed 521118629
Methylated C's in CpG context 6813705
Methylated C's in CHG context 441954
Methylated C's in CHH context 1898020
Unmethylated C's in CpG context 59199373
Unmethylated C's in CHG context 89987061
Unmethylated C's in CHH context 362778516
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot