Bismark report for: /gscratch/srlab/sr320/data/cg/zr3644_22_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_22_R2.fastp-trim.20201206.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/Crassostrea_gigas.oyster_v9.dna_sm.toplevel/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 46739729 Number of paired-end alignments with a unique best hit: 29199933 Mapping efficiency: 62.5% Sequence pairs with no alignments under any condition: 11619522 Sequence pairs did not map uniquely: 5920274 Sequence pairs which were discarded because genomic sequence could not be extracted: 3686 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 7267812 ((converted) top strand) GA/CT/CT: 7319098 (complementary to (converted) top strand) GA/CT/GA: 7328911 (complementary to (converted) bottom strand) CT/GA/GA: 7280426 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1033185635 Total methylated C's in CpG context: 13987572 Total methylated C's in CHG context: 892804 Total methylated C's in CHH context: 3794249 Total methylated C's in Unknown context: 32727 Total unmethylated C's in CpG context: 117946449 Total unmethylated C's in CHG context: 180567038 Total unmethylated C's in CHH context: 715997523 Total unmethylated C's in Unknown context: 2544881 C methylated in CpG context: 10.6% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.3% Bismark completed in 0d 4h 32m 28s