Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_22_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_22_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 46739729
Paired-end alignments with a unique best hit 29199933
Pairs without alignments under any condition 11619522
Pairs that did not map uniquely 5920274
Genomic sequence context not extractable (edges of chromosomes) 3686

Cytosine Methylation

Total C's analysed 1033185635
Methylated C's in CpG context 13987572
Methylated C's in CHG context 892804
Methylated C's in CHH context 3794249
Methylated C's in Unknown context 32727
Unmethylated C's in CpG context 117946449
Unmethylated C's in CHG context 180567038
Unmethylated C's in CHH context 715997523
Unmethylated C's in Unknown context 2544881
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7267812 original top strand
CTOT 7319098 complementary to original top strand
CTOB 7328911 complementary to original bottom strand
OB 7280426 original bottom strand

Deduplication

Alignments analysed 29196247
Unique alignments 25773614
Duplicates removed 3422633
Duplicated alignments were found at 3003877 different positions

Cytosine Methylation after Extraction

Total C's analysed 625977165
Methylated C's in CpG context 8282246
Methylated C's in CHG context 534667
Methylated C's in CHH context 2276014
Unmethylated C's in CpG context 71021427
Unmethylated C's in CHG context 108447960
Unmethylated C's in CHH context 435414851
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot