Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_21_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_21_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 50165026
Paired-end alignments with a unique best hit 30975420
Pairs without alignments under any condition 13023018
Pairs that did not map uniquely 6166588
Genomic sequence context not extractable (edges of chromosomes) 3880

Cytosine Methylation

Total C's analysed 1090983911
Methylated C's in CpG context 14756465
Methylated C's in CHG context 928798
Methylated C's in CHH context 3950640
Methylated C's in Unknown context 35940
Unmethylated C's in CpG context 123917132
Unmethylated C's in CHG context 189121701
Unmethylated C's in CHH context 758309175
Unmethylated C's in Unknown context 2725651
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7748533 original top strand
CTOT 7741135 complementary to original top strand
CTOB 7736552 complementary to original bottom strand
OB 7745320 original bottom strand

Deduplication

Alignments analysed 30971540
Unique alignments 27363020
Duplicates removed 3608520
Duplicated alignments were found at 3174318 different positions

Cytosine Methylation after Extraction

Total C's analysed 668808705
Methylated C's in CpG context 8829172
Methylated C's in CHG context 562477
Methylated C's in CHH context 2398853
Unmethylated C's in CpG context 75508229
Unmethylated C's in CHG context 114890165
Unmethylated C's in CHH context 466619809
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot