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Alignment Stats

Sequence pairs analysed in total 50165026 Paired-end alignments with a unique best hit 30975420 Pairs without alignments under any condition 13023018 Pairs that did not map uniquely 6166588 Genomic sequence context not extractable (edges of chromosomes) 3880

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Cytosine Methylation

Total C's analysed 1090983911 Methylated C's in CpG context 14756465 Methylated C's in CHG context 928798 Methylated C's in CHH context 3950640 Methylated C's in Unknown context 35940 Unmethylated C's in CpG context 123917132 Unmethylated C's in CHG context 189121701 Unmethylated C's in CHH context 758309175 Unmethylated C's in Unknown context 2725651 Percentage methylation (CpG context) 10.6% Percentage methylation (CHG context) 0.5% Percentage methylation (CHH context) 0.5% Methylated C's in Unknown context N/A%

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Alignment to Individual Bisulfite Strands

OT 7748533 original top strand CTOT 7741135 complementary to original top strand CTOB 7736552 complementary to original bottom strand OB 7745320 original bottom strand

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Deduplication

Alignments analysed 30971540 Unique alignments 27363020 Duplicates removed 3608520 Duplicated alignments were found at 3174318 different positions

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Cytosine Methylation after Extraction

Total C's analysed 668808705 Methylated C's in CpG context 8829172 Methylated C's in CHG context 562477 Methylated C's in CHH context 2398853 Unmethylated C's in CpG context 75508229 Unmethylated C's in CHG context 114890165 Unmethylated C's in CHH context 466619809 Percentage methylation (CpG context) 10.5% Percentage methylation (CHG context) 0.5% Percentage methylation (CHH context) 0.5%

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M-Bias Plot

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