Bismark report for: /gscratch/srlab/sr320/data/cg/zr3644_20_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_20_R2.fastp-trim.20201206.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/Crassostrea_gigas.oyster_v9.dna_sm.toplevel/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 53149153 Number of paired-end alignments with a unique best hit: 32579954 Mapping efficiency: 61.3% Sequence pairs with no alignments under any condition: 14142974 Sequence pairs did not map uniquely: 6426225 Sequence pairs which were discarded because genomic sequence could not be extracted: 3807 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 7835017 ((converted) top strand) GA/CT/CT: 8443279 (complementary to (converted) top strand) GA/CT/GA: 8449686 (complementary to (converted) bottom strand) CT/GA/GA: 7848165 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1149666784 Total methylated C's in CpG context: 15395681 Total methylated C's in CHG context: 993551 Total methylated C's in CHH context: 4206864 Total methylated C's in Unknown context: 38277 Total unmethylated C's in CpG context: 130582681 Total unmethylated C's in CHG context: 199240427 Total unmethylated C's in CHH context: 799247580 Total unmethylated C's in Unknown context: 2823577 C methylated in CpG context: 10.5% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.3% Bismark completed in 0d 5h 5m 18s