Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_20_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_20_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 53149153
Paired-end alignments with a unique best hit 32579954
Pairs without alignments under any condition 14142974
Pairs that did not map uniquely 6426225
Genomic sequence context not extractable (edges of chromosomes) 3807

Cytosine Methylation

Total C's analysed 1149666784
Methylated C's in CpG context 15395681
Methylated C's in CHG context 993551
Methylated C's in CHH context 4206864
Methylated C's in Unknown context 38277
Unmethylated C's in CpG context 130582681
Unmethylated C's in CHG context 199240427
Unmethylated C's in CHH context 799247580
Unmethylated C's in Unknown context 2823577
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7835017 original top strand
CTOT 8443279 complementary to original top strand
CTOB 8449686 complementary to original bottom strand
OB 7848165 original bottom strand

Deduplication

Alignments analysed 32576147
Unique alignments 28634525
Duplicates removed 3941622
Duplicated alignments were found at 3459886 different positions

Cytosine Methylation after Extraction

Total C's analysed 702973774
Methylated C's in CpG context 9195073
Methylated C's in CHG context 603531
Methylated C's in CHH context 2560469
Unmethylated C's in CpG context 79458401
Unmethylated C's in CHG context 120801218
Unmethylated C's in CHH context 490355082
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot