Bismark report for: /gscratch/srlab/sr320/data/cg/zr3644_1_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_1_R2.fastp-trim.20201206.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/Crassostrea_gigas.oyster_v9.dna_sm.toplevel/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 50613363 Number of paired-end alignments with a unique best hit: 31733639 Mapping efficiency: 62.7% Sequence pairs with no alignments under any condition: 12202916 Sequence pairs did not map uniquely: 6676808 Sequence pairs which were discarded because genomic sequence could not be extracted: 3659 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 7979296 ((converted) top strand) GA/CT/CT: 7883292 (complementary to (converted) top strand) GA/CT/GA: 7883674 (complementary to (converted) bottom strand) CT/GA/GA: 7983718 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1112435555 Total methylated C's in CpG context: 13822220 Total methylated C's in CHG context: 906946 Total methylated C's in CHH context: 3907246 Total methylated C's in Unknown context: 33720 Total unmethylated C's in CpG context: 127230281 Total unmethylated C's in CHG context: 193885510 Total unmethylated C's in CHH context: 772683352 Total unmethylated C's in Unknown context: 2727121 C methylated in CpG context: 9.8% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.2% Bismark completed in 0d 4h 55m 53s