Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_1_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_1_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 50613363
Paired-end alignments with a unique best hit 31733639
Pairs without alignments under any condition 12202916
Pairs that did not map uniquely 6676808
Genomic sequence context not extractable (edges of chromosomes) 3659

Cytosine Methylation

Total C's analysed 1112435555
Methylated C's in CpG context 13822220
Methylated C's in CHG context 906946
Methylated C's in CHH context 3907246
Methylated C's in Unknown context 33720
Unmethylated C's in CpG context 127230281
Unmethylated C's in CHG context 193885510
Unmethylated C's in CHH context 772683352
Unmethylated C's in Unknown context 2727121
Percentage methylation (CpG context) 9.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7979296 original top strand
CTOT 7883292 complementary to original top strand
CTOB 7883674 complementary to original bottom strand
OB 7983718 original bottom strand

Deduplication

Alignments analysed 31729980
Unique alignments 27761215
Duplicates removed 3968765
Duplicated alignments were found at 3452813 different positions

Cytosine Methylation after Extraction

Total C's analysed 652509614
Methylated C's in CpG context 7936786
Methylated C's in CHG context 525710
Methylated C's in CHH context 2275329
Unmethylated C's in CpG context 74140001
Unmethylated C's in CHG context 112783027
Unmethylated C's in CHH context 454848761
Percentage methylation (CpG context) 9.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot