Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_19_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_19_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 48336315
Paired-end alignments with a unique best hit 30090769
Pairs without alignments under any condition 12162908
Pairs that did not map uniquely 6082638
Genomic sequence context not extractable (edges of chromosomes) 3618

Cytosine Methylation

Total C's analysed 1055910315
Methylated C's in CpG context 14045904
Methylated C's in CHG context 897929
Methylated C's in CHH context 3871260
Methylated C's in Unknown context 33830
Unmethylated C's in CpG context 119967807
Unmethylated C's in CHG context 183401573
Unmethylated C's in CHH context 733725842
Unmethylated C's in Unknown context 2622955
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7519144 original top strand
CTOT 7526589 complementary to original top strand
CTOB 7526616 complementary to original bottom strand
OB 7514802 original bottom strand

Deduplication

Alignments analysed 30087151
Unique alignments 26856127
Duplicates removed 3231024
Duplicated alignments were found at 2852076 different positions

Cytosine Methylation after Extraction

Total C's analysed 649151629
Methylated C's in CpG context 8434745
Methylated C's in CHG context 544466
Methylated C's in CHH context 2354921
Unmethylated C's in CpG context 73351172
Unmethylated C's in CHG context 111793257
Unmethylated C's in CHH context 452673068
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot