Bismark report for: /gscratch/srlab/sr320/data/cg/zr3644_16_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_16_R2.fastp-trim.20201206.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/Crassostrea_gigas.oyster_v9.dna_sm.toplevel/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 50134077 Number of paired-end alignments with a unique best hit: 31344702 Mapping efficiency: 62.5% Sequence pairs with no alignments under any condition: 12085635 Sequence pairs did not map uniquely: 6703740 Sequence pairs which were discarded because genomic sequence could not be extracted: 3712 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 7821783 ((converted) top strand) GA/CT/CT: 7841389 (complementary to (converted) top strand) GA/CT/GA: 7849611 (complementary to (converted) bottom strand) CT/GA/GA: 7828207 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1088871217 Total methylated C's in CpG context: 14928270 Total methylated C's in CHG context: 917631 Total methylated C's in CHH context: 3921327 Total methylated C's in Unknown context: 36292 Total unmethylated C's in CpG context: 123938584 Total unmethylated C's in CHG context: 189497153 Total unmethylated C's in CHH context: 755668252 Total unmethylated C's in Unknown context: 2681441 C methylated in CpG context: 10.8% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.3% Bismark completed in 0d 4h 51m 48s