Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_16_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_16_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 50134077
Paired-end alignments with a unique best hit 31344702
Pairs without alignments under any condition 12085635
Pairs that did not map uniquely 6703740
Genomic sequence context not extractable (edges of chromosomes) 3712

Cytosine Methylation

Total C's analysed 1088871217
Methylated C's in CpG context 14928270
Methylated C's in CHG context 917631
Methylated C's in CHH context 3921327
Methylated C's in Unknown context 36292
Unmethylated C's in CpG context 123938584
Unmethylated C's in CHG context 189497153
Unmethylated C's in CHH context 755668252
Unmethylated C's in Unknown context 2681441
Percentage methylation (CpG context) 10.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7821783 original top strand
CTOT 7841389 complementary to original top strand
CTOB 7849611 complementary to original bottom strand
OB 7828207 original bottom strand

Deduplication

Alignments analysed 31340990
Unique alignments 27465809
Duplicates removed 3875181
Duplicated alignments were found at 3384679 different positions

Cytosine Methylation after Extraction

Total C's analysed 630642910
Methylated C's in CpG context 8455671
Methylated C's in CHG context 525816
Methylated C's in CHH context 2264120
Unmethylated C's in CpG context 71325293
Unmethylated C's in CHG context 108801283
Unmethylated C's in CHH context 439270727
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot