Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_15_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_15_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 58102004
Paired-end alignments with a unique best hit 36100179
Pairs without alignments under any condition 14852748
Pairs that did not map uniquely 7149077
Genomic sequence context not extractable (edges of chromosomes) 4297

Cytosine Methylation

Total C's analysed 1270966403
Methylated C's in CpG context 18346891
Methylated C's in CHG context 1100305
Methylated C's in CHH context 4731062
Methylated C's in Unknown context 44588
Unmethylated C's in CpG context 142823649
Unmethylated C's in CHG context 219609453
Unmethylated C's in CHH context 884355043
Unmethylated C's in Unknown context 3189323
Percentage methylation (CpG context) 11.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 9028133 original top strand
CTOT 9015692 complementary to original top strand
CTOB 9020563 complementary to original bottom strand
OB 9031494 original bottom strand

Deduplication

Alignments analysed 36095882
Unique alignments 31774577
Duplicates removed 4321305
Duplicated alignments were found at 3784438 different positions

Cytosine Methylation after Extraction

Total C's analysed 768281077
Methylated C's in CpG context 10842135
Methylated C's in CHG context 657578
Methylated C's in CHH context 2833118
Unmethylated C's in CpG context 85902809
Unmethylated C's in CHG context 131629506
Unmethylated C's in CHH context 536415931
Percentage methylation (CpG context) 11.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot