Bismark report for: /gscratch/srlab/sr320/data/cg/zr3644_13_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_13_R2.fastp-trim.20201206.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/Crassostrea_gigas.oyster_v9.dna_sm.toplevel/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 52950721 Number of paired-end alignments with a unique best hit: 32796899 Mapping efficiency: 61.9% Sequence pairs with no alignments under any condition: 13651711 Sequence pairs did not map uniquely: 6502111 Sequence pairs which were discarded because genomic sequence could not be extracted: 4307 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8192706 ((converted) top strand) GA/CT/CT: 8193157 (complementary to (converted) top strand) GA/CT/GA: 8206126 (complementary to (converted) bottom strand) CT/GA/GA: 8200603 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1159185195 Total methylated C's in CpG context: 15626981 Total methylated C's in CHG context: 989548 Total methylated C's in CHH context: 4243671 Total methylated C's in Unknown context: 38097 Total unmethylated C's in CpG context: 130883638 Total unmethylated C's in CHG context: 200433137 Total unmethylated C's in CHH context: 807008220 Total unmethylated C's in Unknown context: 2904211 C methylated in CpG context: 10.7% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.3% Bismark completed in 0d 5h 9m 8s