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Alignment Stats

Sequence pairs analysed in total 52950721 Paired-end alignments with a unique best hit 32796899 Pairs without alignments under any condition 13651711 Pairs that did not map uniquely 6502111 Genomic sequence context not extractable (edges of chromosomes) 4307

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Cytosine Methylation

Total C's analysed 1159185195 Methylated C's in CpG context 15626981 Methylated C's in CHG context 989548 Methylated C's in CHH context 4243671 Methylated C's in Unknown context 38097 Unmethylated C's in CpG context 130883638 Unmethylated C's in CHG context 200433137 Unmethylated C's in CHH context 807008220 Unmethylated C's in Unknown context 2904211 Percentage methylation (CpG context) 10.7% Percentage methylation (CHG context) 0.5% Percentage methylation (CHH context) 0.5% Methylated C's in Unknown context N/A%

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Alignment to Individual Bisulfite Strands

OT 8192706 original top strand CTOT 8193157 complementary to original top strand CTOB 8206126 complementary to original bottom strand OB 8200603 original bottom strand

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Deduplication

Alignments analysed 32792592 Unique alignments 28813362 Duplicates removed 3979230 Duplicated alignments were found at 3481268 different positions

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Cytosine Methylation after Extraction

Total C's analysed 706165084 Methylated C's in CpG context 9316772 Methylated C's in CHG context 595443 Methylated C's in CHH context 2559208 Unmethylated C's in CpG context 79271442 Unmethylated C's in CHG context 121088433 Unmethylated C's in CHH context 493333786 Percentage methylation (CpG context) 10.5% Percentage methylation (CHG context) 0.5% Percentage methylation (CHH context) 0.5%

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M-Bias Plot

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