Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_13_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_13_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 52950721
Paired-end alignments with a unique best hit 32796899
Pairs without alignments under any condition 13651711
Pairs that did not map uniquely 6502111
Genomic sequence context not extractable (edges of chromosomes) 4307

Cytosine Methylation

Total C's analysed 1159185195
Methylated C's in CpG context 15626981
Methylated C's in CHG context 989548
Methylated C's in CHH context 4243671
Methylated C's in Unknown context 38097
Unmethylated C's in CpG context 130883638
Unmethylated C's in CHG context 200433137
Unmethylated C's in CHH context 807008220
Unmethylated C's in Unknown context 2904211
Percentage methylation (CpG context) 10.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8192706 original top strand
CTOT 8193157 complementary to original top strand
CTOB 8206126 complementary to original bottom strand
OB 8200603 original bottom strand

Deduplication

Alignments analysed 32792592
Unique alignments 28813362
Duplicates removed 3979230
Duplicated alignments were found at 3481268 different positions

Cytosine Methylation after Extraction

Total C's analysed 706165084
Methylated C's in CpG context 9316772
Methylated C's in CHG context 595443
Methylated C's in CHH context 2559208
Unmethylated C's in CpG context 79271442
Unmethylated C's in CHG context 121088433
Unmethylated C's in CHH context 493333786
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot