Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_12_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_12_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 46634543
Paired-end alignments with a unique best hit 29003146
Pairs without alignments under any condition 11943464
Pairs that did not map uniquely 5687933
Genomic sequence context not extractable (edges of chromosomes) 3106

Cytosine Methylation

Total C's analysed 1012845722
Methylated C's in CpG context 12471833
Methylated C's in CHG context 803989
Methylated C's in CHH context 3447972
Methylated C's in Unknown context 31058
Unmethylated C's in CpG context 113531079
Unmethylated C's in CHG context 170581064
Unmethylated C's in CHH context 712009785
Unmethylated C's in Unknown context 2618222
Percentage methylation (CpG context) 9.9%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7225443 original top strand
CTOT 7291497 complementary to original top strand
CTOB 7268237 complementary to original bottom strand
OB 7214863 original bottom strand

Deduplication

Alignments analysed 29000040
Unique alignments 25709252
Duplicates removed 3290788
Duplicated alignments were found at 2912852 different positions

Cytosine Methylation after Extraction

Total C's analysed 610219881
Methylated C's in CpG context 7329386
Methylated C's in CHG context 479331
Methylated C's in CHH context 2066164
Unmethylated C's in CpG context 68031292
Unmethylated C's in CHG context 101907222
Unmethylated C's in CHH context 430406486
Percentage methylation (CpG context) 9.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot