Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3644_10_R1.fastp-trim.20201206.fq.gz and /gscratch/srlab/sr320/data/cg/zr3644_10_R2.fastp-trim.20201206.fq.gz

Data processed at 15:03 on 2021-02-23


Alignment Stats

Sequence pairs analysed in total 51598026
Paired-end alignments with a unique best hit 31760576
Pairs without alignments under any condition 13253207
Pairs that did not map uniquely 6584243
Genomic sequence context not extractable (edges of chromosomes) 4473

Cytosine Methylation

Total C's analysed 1118051595
Methylated C's in CpG context 15758178
Methylated C's in CHG context 948700
Methylated C's in CHH context 4102459
Methylated C's in Unknown context 39023
Unmethylated C's in CpG context 127363683
Unmethylated C's in CHG context 195086907
Unmethylated C's in CHH context 774791668
Unmethylated C's in Unknown context 2783347
Percentage methylation (CpG context) 11.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7978000 original top strand
CTOT 7907728 complementary to original top strand
CTOB 7901326 complementary to original bottom strand
OB 7969049 original bottom strand

Deduplication

Alignments analysed 31756103
Unique alignments 27938619
Duplicates removed 3817484
Duplicated alignments were found at 3335957 different positions

Cytosine Methylation after Extraction

Total C's analysed 669818145
Methylated C's in CpG context 9224758
Methylated C's in CHG context 561298
Methylated C's in CHH context 2432892
Unmethylated C's in CpG context 75772675
Unmethylated C's in CHG context 115809548
Unmethylated C's in CHH context 466016974
Percentage methylation (CpG context) 10.9%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot