Bismark report for: /gscratch/srlab/sr320/data/cvirg/76F_R1_val_1.fq.gz and /gscratch/srlab/sr320/data/cvirg/76F_R2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cvirg-genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 155271055 Number of paired-end alignments with a unique best hit: 91161529 Mapping efficiency: 58.7% Sequence pairs with no alignments under any condition: 41173079 Sequence pairs did not map uniquely: 22936447 Sequence pairs which were discarded because genomic sequence could not be extracted: 1230 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 23055743 ((converted) top strand) GA/CT/CT: 22910539 (complementary to (converted) top strand) GA/CT/GA: 22526464 (complementary to (converted) bottom strand) CT/GA/GA: 22667553 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 3862098313 Total methylated C's in CpG context: 60581431 Total methylated C's in CHG context: 2720254 Total methylated C's in CHH context: 12090716 Total methylated C's in Unknown context: 271187 Total unmethylated C's in CpG context: 441252446 Total unmethylated C's in CHG context: 691448235 Total unmethylated C's in CHH context: 2654005231 Total unmethylated C's in Unknown context: 12231242 C methylated in CpG context: 12.1% C methylated in CHG context: 0.4% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 2.2% Bismark completed in 0d 15h 50m 4s