Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_all_s456_trimmed.fq

Data processed at 02:25 on 2020-02-05


Alignment Stats

Sequences analysed in total 605809374
Single-end alignments with a unique best hit 185847297
Sequences without alignments under any condition 176357696
Sequences that did not map uniquely 243604381
Genomic sequence context not extractable (edges of chromosomes) 188442

Cytosine Methylation

Total C's analysed 1155350419
Methylated C's in CpG context 175386903
Methylated C's in CHG context 5220316
Methylated C's in CHH context 61488338
Methylated C's in Unknown context 32115
Unmethylated C's in CpG context 30952288
Unmethylated C's in CHG context 231484923
Unmethylated C's in CHH context 650817651
Unmethylated C's in Unknown context 213338
Percentage methylation (CpG context) 85.0%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 8.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 42572282 original top strand
CTOT 50633709 complementary to original top strand
CTOB 50202213 complementary to original bottom strand
OB 42250651 original bottom strand

Deduplication

Alignments analysed 185658855
Unique alignments 61397782
Duplicates removed 124261073
Duplicated alignments were found at 20887234 different positions

Cytosine Methylation after Extraction

Total C's analysed 366293238
Methylated C's in CpG context 37861620
Methylated C's in CHG context 2119087
Methylated C's in CHH context 31889543
Unmethylated C's in CpG context 11160761
Unmethylated C's in CHG context 61988385
Unmethylated C's in CHH context 221273842
Percentage methylation (CpG context) 77.2%
Percentage methylation (CHG context) 3.3%
Percentage methylation (CHH context) 12.6%

M-Bias Plot