Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_9_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-genomes/v081/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 77350922 Number of alignments with a unique best hit from the different alignments: 25389646 Mapping efficiency: 32.8% Sequences with no alignments under any condition: 21586770 Sequences did not map uniquely: 30374506 Sequences which were discarded because genomic sequence could not be extracted: 21762 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 5701971 ((converted) top strand) CT/GA: 5673126 ((converted) bottom strand) GA/CT: 7018609 (complementary to (converted) top strand) GA/GA: 6974178 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 150881047 Total methylated C's in CpG context: 16742773 Total methylated C's in CHG context: 575469 Total methylated C's in CHH context: 8126479 Total methylated C's in Unknown context: 3311 Total unmethylated C's in CpG context: 4068096 Total unmethylated C's in CHG context: 29027906 Total unmethylated C's in CHH context: 92340324 Total unmethylated C's in Unknown context: 25961 C methylated in CpG context: 80.5% C methylated in CHG context: 1.9% C methylated in CHH context: 8.1% C methylated in Unknown context (CN or CHN): 11.3% Bismark completed in 0d 1h 44m 50s