Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_9_s456_trimmed.fq

Data processed at 02:25 on 2020-02-05


Alignment Stats

Sequences analysed in total 77350922
Single-end alignments with a unique best hit 25389646
Sequences without alignments under any condition 21586770
Sequences that did not map uniquely 30374506
Genomic sequence context not extractable (edges of chromosomes) 21762

Cytosine Methylation

Total C's analysed 150881047
Methylated C's in CpG context 16742773
Methylated C's in CHG context 575469
Methylated C's in CHH context 8126479
Methylated C's in Unknown context 3311
Unmethylated C's in CpG context 4068096
Unmethylated C's in CHG context 29027906
Unmethylated C's in CHH context 92340324
Unmethylated C's in Unknown context 25961
Percentage methylation (CpG context) 80.5%
Percentage methylation (CHG context) 1.9%
Percentage methylation (CHH context) 8.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5701971 original top strand
CTOT 7018609 complementary to original top strand
CTOB 6974178 complementary to original bottom strand
OB 5673126 original bottom strand

Deduplication

Alignments analysed 25367884
Unique alignments 16680265
Duplicates removed 8687619
Duplicated alignments were found at 3238822 different positions

Cytosine Methylation after Extraction

Total C's analysed 97723805
Methylated C's in CpG context 9596337
Methylated C's in CHG context 406190
Methylated C's in CHH context 6301142
Unmethylated C's in CpG context 2711789
Unmethylated C's in CHG context 17840843
Unmethylated C's in CHH context 60867504
Percentage methylation (CpG context) 78.0%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 9.4%

M-Bias Plot