Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_7_s456_trimmed.fq

Data processed at 02:25 on 2020-02-05


Alignment Stats

Sequences analysed in total 49730113
Single-end alignments with a unique best hit 15462117
Sequences without alignments under any condition 14857626
Sequences that did not map uniquely 19410370
Genomic sequence context not extractable (edges of chromosomes) 14818

Cytosine Methylation

Total C's analysed 94652195
Methylated C's in CpG context 12800766
Methylated C's in CHG context 441731
Methylated C's in CHH context 5214036
Methylated C's in Unknown context 2528
Unmethylated C's in CpG context 2513676
Unmethylated C's in CHG context 18777732
Unmethylated C's in CHH context 54904254
Unmethylated C's in Unknown context 17310
Percentage methylation (CpG context) 83.6%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 8.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3528969 original top strand
CTOT 4227492 complementary to original top strand
CTOB 4186984 complementary to original bottom strand
OB 3503854 original bottom strand

Deduplication

Alignments analysed 15447299
Unique alignments 10765743
Duplicates removed 4681556
Duplicated alignments were found at 1888964 different positions

Cytosine Methylation after Extraction

Total C's analysed 64930647
Methylated C's in CpG context 7871119
Methylated C's in CHG context 320635
Methylated C's in CHH context 4170941
Unmethylated C's in CpG context 1765508
Unmethylated C's in CHG context 12298987
Unmethylated C's in CHH context 38503457
Percentage methylation (CpG context) 81.7%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 9.8%

M-Bias Plot