Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_6_s456_trimmed.fq

Data processed at 02:25 on 2020-02-05


Alignment Stats

Sequences analysed in total 46455148
Single-end alignments with a unique best hit 15623039
Sequences without alignments under any condition 13764452
Sequences that did not map uniquely 17067657
Genomic sequence context not extractable (edges of chromosomes) 12143

Cytosine Methylation

Total C's analysed 91279017
Methylated C's in CpG context 9068779
Methylated C's in CHG context 406529
Methylated C's in CHH context 5393062
Methylated C's in Unknown context 1851
Unmethylated C's in CpG context 2345574
Unmethylated C's in CHG context 17647340
Unmethylated C's in CHH context 56417733
Unmethylated C's in Unknown context 15035
Percentage methylation (CpG context) 79.5%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 8.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3476758 original top strand
CTOT 4353366 complementary to original top strand
CTOB 4320773 complementary to original bottom strand
OB 3459999 original bottom strand

Deduplication

Alignments analysed 15610896
Unique alignments 11898674
Duplicates removed 3712222
Duplicated alignments were found at 1790136 different positions

Cytosine Methylation after Extraction

Total C's analysed 68879669
Methylated C's in CpG context 6320809
Methylated C's in CHG context 308317
Methylated C's in CHH context 4429364
Unmethylated C's in CpG context 1817405
Unmethylated C's in CHG context 12911503
Unmethylated C's in CHH context 43092271
Percentage methylation (CpG context) 77.7%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 9.3%

M-Bias Plot