Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_5_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-genomes/v081/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 47401736 Number of alignments with a unique best hit from the different alignments: 15838795 Mapping efficiency: 33.4% Sequences with no alignments under any condition: 13874948 Sequences did not map uniquely: 17687993 Sequences which were discarded because genomic sequence could not be extracted: 13185 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3592897 ((converted) top strand) CT/GA: 3571645 ((converted) bottom strand) GA/CT: 4352516 (complementary to (converted) top strand) GA/GA: 4308552 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 93616428 Total methylated C's in CpG context: 9995135 Total methylated C's in CHG context: 402878 Total methylated C's in CHH context: 5641348 Total methylated C's in Unknown context: 2165 Total unmethylated C's in CpG context: 3094855 Total unmethylated C's in CHG context: 18627231 Total unmethylated C's in CHH context: 55854981 Total unmethylated C's in Unknown context: 15434 C methylated in CpG context: 76.4% C methylated in CHG context: 2.1% C methylated in CHH context: 9.2% C methylated in Unknown context (CN or CHN): 12.3% Bismark completed in 0d 1h 5m 8s