Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_5_s456_trimmed.fq

Data processed at 02:25 on 2020-02-05


Alignment Stats

Sequences analysed in total 47401736
Single-end alignments with a unique best hit 15838795
Sequences without alignments under any condition 13874948
Sequences that did not map uniquely 17687993
Genomic sequence context not extractable (edges of chromosomes) 13185
33.4%29.3%37.3%0.0278%
Unique AlignmentsNo AlignmentMultiple AlignmentsNo Genomic Sequence

Cytosine Methylation

Total C's analysed 93616428
Methylated C's in CpG context 9995135
Methylated C's in CHG context 402878
Methylated C's in CHH context 5641348
Methylated C's in Unknown context 2165
Unmethylated C's in CpG context 3094855
Unmethylated C's in CHG context 18627231
Unmethylated C's in CHH context 55854981
Unmethylated C's in Unknown context 15434
Percentage methylation (CpG context) 76.4%
Percentage methylation (CHG context) 2.1%
Percentage methylation (CHH context) 9.2%
Methylated C's in Unknown context N/A%
CpG contextCHG contextCHH context020406080100
% Methylation

Alignment to Individual Bisulfite Strands

OT 3592897 original top strand
CTOT 4352516 complementary to original top strand
CTOB 4308552 complementary to original bottom strand
OB 3571645 original bottom strand
OTCTOTCTOBOB00.5M1M1.5M2M2.5M3M3.5M4M4.5M
Number of Alignments

Deduplication

Alignments analysed 15825610
Unique alignments 11859086
Duplicates removed 3966524
Duplicated alignments were found at 1843603 different positions
74.9%25.1%
Unique AlignmentsDuplicate Alignments

Cytosine Methylation after Extraction

Total C's analysed 69420697
Methylated C's in CpG context 6725035
Methylated C's in CHG context 308623
Methylated C's in CHH context 4618857
Unmethylated C's in CpG context 2383446
Unmethylated C's in CHG context 13280520
Unmethylated C's in CHH context 42104216
Percentage methylation (CpG context) 73.8%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 9.9%
CpG contextCHH contextCHG context020406080100
% Methylation

M-Bias Plot

510152025300204060801000.2M0.4M0.6M0.8M1M1.2M1.4M1.6M
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 1Position in Read [bp]% Methylation# Methylation Calls