Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_4_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-genomes/v081/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 44905078 Number of alignments with a unique best hit from the different alignments: 13945808 Mapping efficiency: 31.1% Sequences with no alignments under any condition: 13481308 Sequences did not map uniquely: 17477962 Sequences which were discarded because genomic sequence could not be extracted: 12622 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3106857 ((converted) top strand) CT/GA: 3082218 ((converted) bottom strand) GA/CT: 3883121 (complementary to (converted) top strand) GA/GA: 3860990 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 85421822 Total methylated C's in CpG context: 11788331 Total methylated C's in CHG context: 407048 Total methylated C's in CHH context: 4800476 Total methylated C's in Unknown context: 2127 Total unmethylated C's in CpG context: 1942535 Total unmethylated C's in CHG context: 16959647 Total unmethylated C's in CHH context: 49523785 Total unmethylated C's in Unknown context: 15252 C methylated in CpG context: 85.9% C methylated in CHG context: 2.3% C methylated in CHH context: 8.8% C methylated in Unknown context (CN or CHN): 12.2% Bismark completed in 0d 0h 59m 31s