Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_4_s456_trimmed.fq

Data processed at 02:25 on 2020-02-05


Alignment Stats

Sequences analysed in total 44905078
Single-end alignments with a unique best hit 13945808
Sequences without alignments under any condition 13481308
Sequences that did not map uniquely 17477962
Genomic sequence context not extractable (edges of chromosomes) 12622

Cytosine Methylation

Total C's analysed 85421822
Methylated C's in CpG context 11788331
Methylated C's in CHG context 407048
Methylated C's in CHH context 4800476
Methylated C's in Unknown context 2127
Unmethylated C's in CpG context 1942535
Unmethylated C's in CHG context 16959647
Unmethylated C's in CHH context 49523785
Unmethylated C's in Unknown context 15252
Percentage methylation (CpG context) 85.9%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 8.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3106857 original top strand
CTOT 3883121 complementary to original top strand
CTOB 3860990 complementary to original bottom strand
OB 3082218 original bottom strand

Deduplication

Alignments analysed 13933186
Unique alignments 9579915
Duplicates removed 4353271
Duplicated alignments were found at 1712445 different positions

Cytosine Methylation after Extraction

Total C's analysed 57797022
Methylated C's in CpG context 7147884
Methylated C's in CHG context 292457
Methylated C's in CHH context 3772656
Unmethylated C's in CpG context 1323418
Unmethylated C's in CHG context 10962379
Unmethylated C's in CHH context 34298228
Percentage methylation (CpG context) 84.4%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 9.9%

M-Bias Plot