Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_3_s456_trimmed.fq

Data processed at 02:25 on 2020-02-05


Alignment Stats

Sequences analysed in total 48376729
Single-end alignments with a unique best hit 14840591
Sequences without alignments under any condition 14694166
Sequences that did not map uniquely 18841972
Genomic sequence context not extractable (edges of chromosomes) 14196

Cytosine Methylation

Total C's analysed 89652817
Methylated C's in CpG context 11470218
Methylated C's in CHG context 407520
Methylated C's in CHH context 5151567
Methylated C's in Unknown context 2367
Unmethylated C's in CpG context 2387780
Unmethylated C's in CHG context 17217824
Unmethylated C's in CHH context 53017908
Unmethylated C's in Unknown context 16543
Percentage methylation (CpG context) 82.8%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 8.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3320140 original top strand
CTOT 4125404 complementary to original top strand
CTOB 4086362 complementary to original bottom strand
OB 3294489 original bottom strand

Deduplication

Alignments analysed 14826395
Unique alignments 10505676
Duplicates removed 4320719
Duplicated alignments were found at 1737411 different positions

Cytosine Methylation after Extraction

Total C's analysed 62586254
Methylated C's in CpG context 7108309
Methylated C's in CHG context 298530
Methylated C's in CHH context 4119726
Unmethylated C's in CpG context 1714938
Unmethylated C's in CHG context 11486929
Unmethylated C's in CHH context 37857822
Percentage methylation (CpG context) 80.6%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 9.8%

M-Bias Plot