Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_2_s456_trimmed.fq

Data processed at 02:25 on 2020-02-05


Alignment Stats

Sequences analysed in total 46105140
Single-end alignments with a unique best hit 14905949
Sequences without alignments under any condition 13855099
Sequences that did not map uniquely 17344092
Genomic sequence context not extractable (edges of chromosomes) 12908

Cytosine Methylation

Total C's analysed 89997267
Methylated C's in CpG context 11472605
Methylated C's in CHG context 410681
Methylated C's in CHH context 5226271
Methylated C's in Unknown context 2218
Unmethylated C's in CpG context 2334664
Unmethylated C's in CHG context 18029891
Unmethylated C's in CHH context 52523155
Unmethylated C's in Unknown context 15400
Percentage methylation (CpG context) 83.1%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 9.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3323802 original top strand
CTOT 4149123 complementary to original top strand
CTOB 4116561 complementary to original bottom strand
OB 3303555 original bottom strand

Deduplication

Alignments analysed 14893041
Unique alignments 10344013
Duplicates removed 4549028
Duplicated alignments were found at 1976360 different positions

Cytosine Methylation after Extraction

Total C's analysed 61756286
Methylated C's in CpG context 7131612
Methylated C's in CHG context 297938
Methylated C's in CHH context 4106610
Unmethylated C's in CpG context 1653180
Unmethylated C's in CHG context 11820461
Unmethylated C's in CHH context 36746485
Percentage methylation (CpG context) 81.2%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 10.1%

M-Bias Plot