Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_1_s456_trimmed.fq

Data processed at 02:25 on 2020-02-05


Alignment Stats

Sequences analysed in total 46792286
Single-end alignments with a unique best hit 14613325
Sequences without alignments under any condition 13908254
Sequences that did not map uniquely 18270707
Genomic sequence context not extractable (edges of chromosomes) 13389

Cytosine Methylation

Total C's analysed 88932661
Methylated C's in CpG context 11765630
Methylated C's in CHG context 420023
Methylated C's in CHH context 5013472
Methylated C's in Unknown context 2315
Unmethylated C's in CpG context 2476805
Unmethylated C's in CHG context 17514384
Unmethylated C's in CHH context 51742347
Unmethylated C's in Unknown context 15939
Percentage methylation (CpG context) 82.6%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 8.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3305147 original top strand
CTOT 4025896 complementary to original top strand
CTOB 3988767 complementary to original bottom strand
OB 3280126 original bottom strand

Deduplication

Alignments analysed 14599936
Unique alignments 10263164
Duplicates removed 4336772
Duplicated alignments were found at 1757896 different positions

Cytosine Methylation after Extraction

Total C's analysed 61540104
Methylated C's in CpG context 7261799
Methylated C's in CHG context 303140
Methylated C's in CHH context 3988762
Unmethylated C's in CpG context 1790508
Unmethylated C's in CHG context 11577501
Unmethylated C's in CHH context 36618394
Percentage methylation (CpG context) 80.2%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 9.8%

M-Bias Plot