Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_18_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-genomes/v081/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 55048106 Number of alignments with a unique best hit from the different alignments: 18170956 Mapping efficiency: 33.0% Sequences with no alignments under any condition: 15404219 Sequences did not map uniquely: 21472931 Sequences which were discarded because genomic sequence could not be extracted: 16272 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3982167 ((converted) top strand) CT/GA: 3955904 ((converted) bottom strand) GA/CT: 5128139 (complementary to (converted) top strand) GA/GA: 5088474 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 109733028 Total methylated C's in CpG context: 14029679 Total methylated C's in CHG context: 448447 Total methylated C's in CHH context: 5906339 Total methylated C's in Unknown context: 2550 Total unmethylated C's in CpG context: 2832392 Total unmethylated C's in CHG context: 22328360 Total unmethylated C's in CHH context: 64187811 Total unmethylated C's in Unknown context: 18670 C methylated in CpG context: 83.2% C methylated in CHG context: 2.0% C methylated in CHH context: 8.4% C methylated in Unknown context (CN or CHN): 12.0% Bismark completed in 0d 1h 14m 26s