Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_17_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-genomes/v081/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 42070546 Number of alignments with a unique best hit from the different alignments: 12889517 Mapping efficiency: 30.6% Sequences with no alignments under any condition: 12141296 Sequences did not map uniquely: 17039733 Sequences which were discarded because genomic sequence could not be extracted: 12594 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2930240 ((converted) top strand) CT/GA: 2910299 ((converted) bottom strand) GA/CT: 3530623 (complementary to (converted) top strand) GA/GA: 3505761 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 80296899 Total methylated C's in CpG context: 12864321 Total methylated C's in CHG context: 385486 Total methylated C's in CHH context: 4484256 Total methylated C's in Unknown context: 2313 Total unmethylated C's in CpG context: 2034662 Total unmethylated C's in CHG context: 16730062 Total unmethylated C's in CHH context: 43798112 Total unmethylated C's in Unknown context: 15331 C methylated in CpG context: 86.3% C methylated in CHG context: 2.3% C methylated in CHH context: 9.3% C methylated in Unknown context (CN or CHN): 13.1% Bismark completed in 0d 0h 55m 52s