Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_17_s456_trimmed.fq

Data processed at 02:25 on 2020-02-05


Alignment Stats

Sequences analysed in total 42070546
Single-end alignments with a unique best hit 12889517
Sequences without alignments under any condition 12141296
Sequences that did not map uniquely 17039733
Genomic sequence context not extractable (edges of chromosomes) 12594

Cytosine Methylation

Total C's analysed 80296899
Methylated C's in CpG context 12864321
Methylated C's in CHG context 385486
Methylated C's in CHH context 4484256
Methylated C's in Unknown context 2313
Unmethylated C's in CpG context 2034662
Unmethylated C's in CHG context 16730062
Unmethylated C's in CHH context 43798112
Unmethylated C's in Unknown context 15331
Percentage methylation (CpG context) 86.3%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 9.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2930240 original top strand
CTOT 3530623 complementary to original top strand
CTOB 3505761 complementary to original bottom strand
OB 2910299 original bottom strand

Deduplication

Alignments analysed 12876923
Unique alignments 7846470
Duplicates removed 5030453
Duplicated alignments were found at 1723416 different positions

Cytosine Methylation after Extraction

Total C's analysed 48108192
Methylated C's in CpG context 6661066
Methylated C's in CHG context 251010
Methylated C's in CHH context 3311685
Unmethylated C's in CpG context 1196534
Unmethylated C's in CHG context 9443102
Unmethylated C's in CHH context 27244795
Percentage methylation (CpG context) 84.8%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 10.8%

M-Bias Plot